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Bio X Cell anti mouse cd28
Mechanistic validation of Ast‐mediated regulation of senescence‐associated CD4 + T cells in aged colitis. (A) Experimental flowchart of NG treatment in aged colitis mice ( n = 6). (B) Representative fecal samples following occult blood testing. (C) Curve chart analysis of DAI ( n = 6). (D) Representative images of colons and corresponding quantification of colon length ( n = 6). (E) Quantification of PD‐1 + CD4 T cells in colonic lamina propria cells ( n = 6). (F) Representative FC plots and quantitative analysis of CD4 + CD27 − <t>CD28</t> − T cells in colonic lamina propria cells ( n = 6). (G) Schematic illustration of the isolation and sorting strategy for senescence‐associated (CD28 − CD27 − ) and non‐senescence‐associated (remaining) CD4 + T cells. Created with BioRender. (H) MFI of PD‐1 expression on senescence‐associated and non‐senescence‐associated CD4 + T cells after treatment with PBS or Ast for 2 days ( n = 3). (I) Cell viability of senescence‐associated and non‐senescence‐associated CD4 + T cells ( n = 3). (J) Foxp3 + T reg differentiation capacity of senescence‐associated and non‐senescence‐associated CD4 + T cells in the presence of PBS or Ast for 2 days ( n = 3). (K) Experimental groups for therapeutic evaluation in aged colitis mice. (L) Curve chart analysis of DAI ( n = 6). (M) Quantification of colon length ( n = 6). Quantitative analysis of (N) CD4 + PD‐1 + T cells and (O) CD4 + CD27 − CD28 − T cells in colonic lamina propria cells ( n = 6). (P) Schematic illustration of CD4 + T cells cultured in the presence of plate‐bound agonistic aPD‐1 or Ast. Created with BioRender. (Q) Quantitative analysis of CD4 + CD27 − CD28 − T cells following treatment with plate‐bound agonistic aPD‐1 or Ast ( n = 3). Data are presented as mean ± SD and analyzed by Two‐way ANOVA with Tukey's post‐test (C, L) or One‐way ANOVA with Tukey's post‐test (D‐F, H‐J, M‐O, Q). ns represents not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.
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anticd3 anti cd28  (Miltenyi Biotec)
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Mechanistic validation of Ast‐mediated regulation of senescence‐associated CD4 + T cells in aged colitis. (A) Experimental flowchart of NG treatment in aged colitis mice ( n = 6). (B) Representative fecal samples following occult blood testing. (C) Curve chart analysis of DAI ( n = 6). (D) Representative images of colons and corresponding quantification of colon length ( n = 6). (E) Quantification of PD‐1 + CD4 T cells in colonic lamina propria cells ( n = 6). (F) Representative FC plots and quantitative analysis of CD4 + CD27 − <t>CD28</t> − T cells in colonic lamina propria cells ( n = 6). (G) Schematic illustration of the isolation and sorting strategy for senescence‐associated (CD28 − CD27 − ) and non‐senescence‐associated (remaining) CD4 + T cells. Created with BioRender. (H) MFI of PD‐1 expression on senescence‐associated and non‐senescence‐associated CD4 + T cells after treatment with PBS or Ast for 2 days ( n = 3). (I) Cell viability of senescence‐associated and non‐senescence‐associated CD4 + T cells ( n = 3). (J) Foxp3 + T reg differentiation capacity of senescence‐associated and non‐senescence‐associated CD4 + T cells in the presence of PBS or Ast for 2 days ( n = 3). (K) Experimental groups for therapeutic evaluation in aged colitis mice. (L) Curve chart analysis of DAI ( n = 6). (M) Quantification of colon length ( n = 6). Quantitative analysis of (N) CD4 + PD‐1 + T cells and (O) CD4 + CD27 − CD28 − T cells in colonic lamina propria cells ( n = 6). (P) Schematic illustration of CD4 + T cells cultured in the presence of plate‐bound agonistic aPD‐1 or Ast. Created with BioRender. (Q) Quantitative analysis of CD4 + CD27 − CD28 − T cells following treatment with plate‐bound agonistic aPD‐1 or Ast ( n = 3). Data are presented as mean ± SD and analyzed by Two‐way ANOVA with Tukey's post‐test (C, L) or One‐way ANOVA with Tukey's post‐test (D‐F, H‐J, M‐O, Q). ns represents not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.
Anticd3 Anti Cd28, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd28  (Bio X Cell)
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Bio X Cell cd28
Mechanistic validation of Ast‐mediated regulation of senescence‐associated CD4 + T cells in aged colitis. (A) Experimental flowchart of NG treatment in aged colitis mice ( n = 6). (B) Representative fecal samples following occult blood testing. (C) Curve chart analysis of DAI ( n = 6). (D) Representative images of colons and corresponding quantification of colon length ( n = 6). (E) Quantification of PD‐1 + CD4 T cells in colonic lamina propria cells ( n = 6). (F) Representative FC plots and quantitative analysis of CD4 + CD27 − <t>CD28</t> − T cells in colonic lamina propria cells ( n = 6). (G) Schematic illustration of the isolation and sorting strategy for senescence‐associated (CD28 − CD27 − ) and non‐senescence‐associated (remaining) CD4 + T cells. Created with BioRender. (H) MFI of PD‐1 expression on senescence‐associated and non‐senescence‐associated CD4 + T cells after treatment with PBS or Ast for 2 days ( n = 3). (I) Cell viability of senescence‐associated and non‐senescence‐associated CD4 + T cells ( n = 3). (J) Foxp3 + T reg differentiation capacity of senescence‐associated and non‐senescence‐associated CD4 + T cells in the presence of PBS or Ast for 2 days ( n = 3). (K) Experimental groups for therapeutic evaluation in aged colitis mice. (L) Curve chart analysis of DAI ( n = 6). (M) Quantification of colon length ( n = 6). Quantitative analysis of (N) CD4 + PD‐1 + T cells and (O) CD4 + CD27 − CD28 − T cells in colonic lamina propria cells ( n = 6). (P) Schematic illustration of CD4 + T cells cultured in the presence of plate‐bound agonistic aPD‐1 or Ast. Created with BioRender. (Q) Quantitative analysis of CD4 + CD27 − CD28 − T cells following treatment with plate‐bound agonistic aPD‐1 or Ast ( n = 3). Data are presented as mean ± SD and analyzed by Two‐way ANOVA with Tukey's post‐test (C, L) or One‐way ANOVA with Tukey's post‐test (D‐F, H‐J, M‐O, Q). ns represents not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.
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cd28 antibody  (Biogems International)
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Biogems International cd28 antibody
Mechanistic validation of Ast‐mediated regulation of senescence‐associated CD4 + T cells in aged colitis. (A) Experimental flowchart of NG treatment in aged colitis mice ( n = 6). (B) Representative fecal samples following occult blood testing. (C) Curve chart analysis of DAI ( n = 6). (D) Representative images of colons and corresponding quantification of colon length ( n = 6). (E) Quantification of PD‐1 + CD4 T cells in colonic lamina propria cells ( n = 6). (F) Representative FC plots and quantitative analysis of CD4 + CD27 − <t>CD28</t> − T cells in colonic lamina propria cells ( n = 6). (G) Schematic illustration of the isolation and sorting strategy for senescence‐associated (CD28 − CD27 − ) and non‐senescence‐associated (remaining) CD4 + T cells. Created with BioRender. (H) MFI of PD‐1 expression on senescence‐associated and non‐senescence‐associated CD4 + T cells after treatment with PBS or Ast for 2 days ( n = 3). (I) Cell viability of senescence‐associated and non‐senescence‐associated CD4 + T cells ( n = 3). (J) Foxp3 + T reg differentiation capacity of senescence‐associated and non‐senescence‐associated CD4 + T cells in the presence of PBS or Ast for 2 days ( n = 3). (K) Experimental groups for therapeutic evaluation in aged colitis mice. (L) Curve chart analysis of DAI ( n = 6). (M) Quantification of colon length ( n = 6). Quantitative analysis of (N) CD4 + PD‐1 + T cells and (O) CD4 + CD27 − CD28 − T cells in colonic lamina propria cells ( n = 6). (P) Schematic illustration of CD4 + T cells cultured in the presence of plate‐bound agonistic aPD‐1 or Ast. Created with BioRender. (Q) Quantitative analysis of CD4 + CD27 − CD28 − T cells following treatment with plate‐bound agonistic aPD‐1 or Ast ( n = 3). Data are presented as mean ± SD and analyzed by Two‐way ANOVA with Tukey's post‐test (C, L) or One‐way ANOVA with Tukey's post‐test (D‐F, H‐J, M‐O, Q). ns represents not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.
Cd28 Antibody, supplied by Biogems International, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti cd28 antibody  (Bio X Cell)
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Bio X Cell anti cd28 antibody
Mechanistic validation of Ast‐mediated regulation of senescence‐associated CD4 + T cells in aged colitis. (A) Experimental flowchart of NG treatment in aged colitis mice ( n = 6). (B) Representative fecal samples following occult blood testing. (C) Curve chart analysis of DAI ( n = 6). (D) Representative images of colons and corresponding quantification of colon length ( n = 6). (E) Quantification of PD‐1 + CD4 T cells in colonic lamina propria cells ( n = 6). (F) Representative FC plots and quantitative analysis of CD4 + CD27 − <t>CD28</t> − T cells in colonic lamina propria cells ( n = 6). (G) Schematic illustration of the isolation and sorting strategy for senescence‐associated (CD28 − CD27 − ) and non‐senescence‐associated (remaining) CD4 + T cells. Created with BioRender. (H) MFI of PD‐1 expression on senescence‐associated and non‐senescence‐associated CD4 + T cells after treatment with PBS or Ast for 2 days ( n = 3). (I) Cell viability of senescence‐associated and non‐senescence‐associated CD4 + T cells ( n = 3). (J) Foxp3 + T reg differentiation capacity of senescence‐associated and non‐senescence‐associated CD4 + T cells in the presence of PBS or Ast for 2 days ( n = 3). (K) Experimental groups for therapeutic evaluation in aged colitis mice. (L) Curve chart analysis of DAI ( n = 6). (M) Quantification of colon length ( n = 6). Quantitative analysis of (N) CD4 + PD‐1 + T cells and (O) CD4 + CD27 − CD28 − T cells in colonic lamina propria cells ( n = 6). (P) Schematic illustration of CD4 + T cells cultured in the presence of plate‐bound agonistic aPD‐1 or Ast. Created with BioRender. (Q) Quantitative analysis of CD4 + CD27 − CD28 − T cells following treatment with plate‐bound agonistic aPD‐1 or Ast ( n = 3). Data are presented as mean ± SD and analyzed by Two‐way ANOVA with Tukey's post‐test (C, L) or One‐way ANOVA with Tukey's post‐test (D‐F, H‐J, M‐O, Q). ns represents not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.
Anti Cd28 Antibody, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibovine cd28 antibody  (Bio-Rad)
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Mechanistic validation of Ast‐mediated regulation of senescence‐associated CD4 + T cells in aged colitis. (A) Experimental flowchart of NG treatment in aged colitis mice ( n = 6). (B) Representative fecal samples following occult blood testing. (C) Curve chart analysis of DAI ( n = 6). (D) Representative images of colons and corresponding quantification of colon length ( n = 6). (E) Quantification of PD‐1 + CD4 T cells in colonic lamina propria cells ( n = 6). (F) Representative FC plots and quantitative analysis of CD4 + CD27 − <t>CD28</t> − T cells in colonic lamina propria cells ( n = 6). (G) Schematic illustration of the isolation and sorting strategy for senescence‐associated (CD28 − CD27 − ) and non‐senescence‐associated (remaining) CD4 + T cells. Created with BioRender. (H) MFI of PD‐1 expression on senescence‐associated and non‐senescence‐associated CD4 + T cells after treatment with PBS or Ast for 2 days ( n = 3). (I) Cell viability of senescence‐associated and non‐senescence‐associated CD4 + T cells ( n = 3). (J) Foxp3 + T reg differentiation capacity of senescence‐associated and non‐senescence‐associated CD4 + T cells in the presence of PBS or Ast for 2 days ( n = 3). (K) Experimental groups for therapeutic evaluation in aged colitis mice. (L) Curve chart analysis of DAI ( n = 6). (M) Quantification of colon length ( n = 6). Quantitative analysis of (N) CD4 + PD‐1 + T cells and (O) CD4 + CD27 − CD28 − T cells in colonic lamina propria cells ( n = 6). (P) Schematic illustration of CD4 + T cells cultured in the presence of plate‐bound agonistic aPD‐1 or Ast. Created with BioRender. (Q) Quantitative analysis of CD4 + CD27 − CD28 − T cells following treatment with plate‐bound agonistic aPD‐1 or Ast ( n = 3). Data are presented as mean ± SD and analyzed by Two‐way ANOVA with Tukey's post‐test (C, L) or One‐way ANOVA with Tukey's post‐test (D‐F, H‐J, M‐O, Q). ns represents not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.
Antibovine Cd28 Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Mechanistic validation of Ast‐mediated regulation of senescence‐associated CD4 + T cells in aged colitis. (A) Experimental flowchart of NG treatment in aged colitis mice ( n = 6). (B) Representative fecal samples following occult blood testing. (C) Curve chart analysis of DAI ( n = 6). (D) Representative images of colons and corresponding quantification of colon length ( n = 6). (E) Quantification of PD‐1 + CD4 T cells in colonic lamina propria cells ( n = 6). (F) Representative FC plots and quantitative analysis of CD4 + CD27 − <t>CD28</t> − T cells in colonic lamina propria cells ( n = 6). (G) Schematic illustration of the isolation and sorting strategy for senescence‐associated (CD28 − CD27 − ) and non‐senescence‐associated (remaining) CD4 + T cells. Created with BioRender. (H) MFI of PD‐1 expression on senescence‐associated and non‐senescence‐associated CD4 + T cells after treatment with PBS or Ast for 2 days ( n = 3). (I) Cell viability of senescence‐associated and non‐senescence‐associated CD4 + T cells ( n = 3). (J) Foxp3 + T reg differentiation capacity of senescence‐associated and non‐senescence‐associated CD4 + T cells in the presence of PBS or Ast for 2 days ( n = 3). (K) Experimental groups for therapeutic evaluation in aged colitis mice. (L) Curve chart analysis of DAI ( n = 6). (M) Quantification of colon length ( n = 6). Quantitative analysis of (N) CD4 + PD‐1 + T cells and (O) CD4 + CD27 − CD28 − T cells in colonic lamina propria cells ( n = 6). (P) Schematic illustration of CD4 + T cells cultured in the presence of plate‐bound agonistic aPD‐1 or Ast. Created with BioRender. (Q) Quantitative analysis of CD4 + CD27 − CD28 − T cells following treatment with plate‐bound agonistic aPD‐1 or Ast ( n = 3). Data are presented as mean ± SD and analyzed by Two‐way ANOVA with Tukey's post‐test (C, L) or One‐way ANOVA with Tukey's post‐test (D‐F, H‐J, M‐O, Q). ns represents not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.
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soluble anti cd28  (ABclonal Biotechnology)
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Mechanistic validation of Ast‐mediated regulation of senescence‐associated CD4 + T cells in aged colitis. (A) Experimental flowchart of NG treatment in aged colitis mice ( n = 6). (B) Representative fecal samples following occult blood testing. (C) Curve chart analysis of DAI ( n = 6). (D) Representative images of colons and corresponding quantification of colon length ( n = 6). (E) Quantification of PD‐1 + CD4 T cells in colonic lamina propria cells ( n = 6). (F) Representative FC plots and quantitative analysis of CD4 + CD27 − <t>CD28</t> − T cells in colonic lamina propria cells ( n = 6). (G) Schematic illustration of the isolation and sorting strategy for senescence‐associated (CD28 − CD27 − ) and non‐senescence‐associated (remaining) CD4 + T cells. Created with BioRender. (H) MFI of PD‐1 expression on senescence‐associated and non‐senescence‐associated CD4 + T cells after treatment with PBS or Ast for 2 days ( n = 3). (I) Cell viability of senescence‐associated and non‐senescence‐associated CD4 + T cells ( n = 3). (J) Foxp3 + T reg differentiation capacity of senescence‐associated and non‐senescence‐associated CD4 + T cells in the presence of PBS or Ast for 2 days ( n = 3). (K) Experimental groups for therapeutic evaluation in aged colitis mice. (L) Curve chart analysis of DAI ( n = 6). (M) Quantification of colon length ( n = 6). Quantitative analysis of (N) CD4 + PD‐1 + T cells and (O) CD4 + CD27 − CD28 − T cells in colonic lamina propria cells ( n = 6). (P) Schematic illustration of CD4 + T cells cultured in the presence of plate‐bound agonistic aPD‐1 or Ast. Created with BioRender. (Q) Quantitative analysis of CD4 + CD27 − CD28 − T cells following treatment with plate‐bound agonistic aPD‐1 or Ast ( n = 3). Data are presented as mean ± SD and analyzed by Two‐way ANOVA with Tukey's post‐test (C, L) or One‐way ANOVA with Tukey's post‐test (D‐F, H‐J, M‐O, Q). ns represents not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.
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Mechanistic validation of Ast‐mediated regulation of senescence‐associated CD4 + T cells in aged colitis. (A) Experimental flowchart of NG treatment in aged colitis mice ( n = 6). (B) Representative fecal samples following occult blood testing. (C) Curve chart analysis of DAI ( n = 6). (D) Representative images of colons and corresponding quantification of colon length ( n = 6). (E) Quantification of PD‐1 + CD4 T cells in colonic lamina propria cells ( n = 6). (F) Representative FC plots and quantitative analysis of CD4 + CD27 − CD28 − T cells in colonic lamina propria cells ( n = 6). (G) Schematic illustration of the isolation and sorting strategy for senescence‐associated (CD28 − CD27 − ) and non‐senescence‐associated (remaining) CD4 + T cells. Created with BioRender. (H) MFI of PD‐1 expression on senescence‐associated and non‐senescence‐associated CD4 + T cells after treatment with PBS or Ast for 2 days ( n = 3). (I) Cell viability of senescence‐associated and non‐senescence‐associated CD4 + T cells ( n = 3). (J) Foxp3 + T reg differentiation capacity of senescence‐associated and non‐senescence‐associated CD4 + T cells in the presence of PBS or Ast for 2 days ( n = 3). (K) Experimental groups for therapeutic evaluation in aged colitis mice. (L) Curve chart analysis of DAI ( n = 6). (M) Quantification of colon length ( n = 6). Quantitative analysis of (N) CD4 + PD‐1 + T cells and (O) CD4 + CD27 − CD28 − T cells in colonic lamina propria cells ( n = 6). (P) Schematic illustration of CD4 + T cells cultured in the presence of plate‐bound agonistic aPD‐1 or Ast. Created with BioRender. (Q) Quantitative analysis of CD4 + CD27 − CD28 − T cells following treatment with plate‐bound agonistic aPD‐1 or Ast ( n = 3). Data are presented as mean ± SD and analyzed by Two‐way ANOVA with Tukey's post‐test (C, L) or One‐way ANOVA with Tukey's post‐test (D‐F, H‐J, M‐O, Q). ns represents not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

Journal: Advanced Science

Article Title: Oral Astilbin Nanogels Modulate Senescence‐Associated CD4 + T Cells and Promote T reg Induction in Aged Mice with Colitis

doi: 10.1002/advs.78123

Figure Lengend Snippet: Mechanistic validation of Ast‐mediated regulation of senescence‐associated CD4 + T cells in aged colitis. (A) Experimental flowchart of NG treatment in aged colitis mice ( n = 6). (B) Representative fecal samples following occult blood testing. (C) Curve chart analysis of DAI ( n = 6). (D) Representative images of colons and corresponding quantification of colon length ( n = 6). (E) Quantification of PD‐1 + CD4 T cells in colonic lamina propria cells ( n = 6). (F) Representative FC plots and quantitative analysis of CD4 + CD27 − CD28 − T cells in colonic lamina propria cells ( n = 6). (G) Schematic illustration of the isolation and sorting strategy for senescence‐associated (CD28 − CD27 − ) and non‐senescence‐associated (remaining) CD4 + T cells. Created with BioRender. (H) MFI of PD‐1 expression on senescence‐associated and non‐senescence‐associated CD4 + T cells after treatment with PBS or Ast for 2 days ( n = 3). (I) Cell viability of senescence‐associated and non‐senescence‐associated CD4 + T cells ( n = 3). (J) Foxp3 + T reg differentiation capacity of senescence‐associated and non‐senescence‐associated CD4 + T cells in the presence of PBS or Ast for 2 days ( n = 3). (K) Experimental groups for therapeutic evaluation in aged colitis mice. (L) Curve chart analysis of DAI ( n = 6). (M) Quantification of colon length ( n = 6). Quantitative analysis of (N) CD4 + PD‐1 + T cells and (O) CD4 + CD27 − CD28 − T cells in colonic lamina propria cells ( n = 6). (P) Schematic illustration of CD4 + T cells cultured in the presence of plate‐bound agonistic aPD‐1 or Ast. Created with BioRender. (Q) Quantitative analysis of CD4 + CD27 − CD28 − T cells following treatment with plate‐bound agonistic aPD‐1 or Ast ( n = 3). Data are presented as mean ± SD and analyzed by Two‐way ANOVA with Tukey's post‐test (C, L) or One‐way ANOVA with Tukey's post‐test (D‐F, H‐J, M‐O, Q). ns represents not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

Article Snippet: Purified blocking anti‐mouse PD‐1 (Clone RMP1‐14), agonistic anti‐mouse PD‐1 (Clone RMP1‐30), anti‐mouse CD3 (Clone 17A2), and anti‐mouse CD28 (Clone 37.51) were purchased from BioXcell (USA).

Techniques: Biomarker Discovery, Fecal/Stool Samples, Analysis, Isolation, Expressing, Cell Culture, FACS